It is increasingly recognized as a serious public health concern worldwide. pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae infection may be associated with treatment failure and increased mortality ( Toledo et al., 2015). It causes a wide range of hospital-acquired infections, such as wound infection, bacteremia, pneumonia, and urinary tract infections, particularly in immunocompromised people ( Rani et al., 2017). Klebsiella pneumoniae is a Gram-negative, encapsulated, non-motile, rod-shaped opportunistic pathogen. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. 4Department of Medicine, Vascular Biology Center, Medical College of Georgia at Augusta University, Augusta, GA, United StatesĬurrently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria.3King Abdullah Institute of Nanotechnology, King Saud University, Riyadh, Saudi Arabia.
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2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.1Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.Alkhulaifi 1, Monerah AlOthman 1, Ibrahim Khalid 2, Elnagar Doaa 2, Khatab Alaa 2, Manal A.